Double Beam UV-VIS Spectrophotometer

Basic Mode :

To measure the Absorbance and Tranmittance

Quantitative :

1. Coefficient Method
   2. Standard Curve Up to 10 Standard sample may be used to establish a curve. Four methods for fitting a curve through the calibration points : Linear fit. Linear fit through zero, Square fit and cubic fit.

Wavelength Scan :

1. The wavelength scan intervals are 0.1,0.2,0.5,1,2 & 5 nm
   2. Fast, Medium and Slow scan speed are available. They vary from 100 to
   3600 nm/min
   3. Wavelength are scanned from high to low so that the instrument waits at high.
   Wavelength and it minimizes the degradation of UV sensitive samples.

Kinetics :

This mode may be used for time course scanning or reaction rate calculations.
Abs vs time graphs is displayed on the screen in real time wait time and
measurement time up to 12 hours may be entered with time interval of
0.5,1,2,5,10,30 seconds and 1 min. Post-run manipulation includes re-scalling,
curve tracking and selection of the part of the curve required for rate calculation.
Rate is calculated using a linear regression algorithm before multiplying be the
entered factor.

DNA / Protein Test :

Concentration and DNA purity are quickly and easily calculated: Absorbance
rations 260 nm / 280 nm with optional subtracted absorbance at 320 nm.
DNA concentration = 62.9XA260-36.0XA280
Protein concentration = 1552xA260-757.3xA 280

Specifications

Design & Specification are subject to change without any prior notice.