The TRUSTwell TSH ELISA Kit is a solid phase enzyme linked immunosorbent assay for the quantitative determination of the levels of Thyroid Stimulating Hormone (TSH) in human serum.
- Sensitivity: Detects TSH levels as low as 0.035 µIU/mL
- Specificity:Â No cross reactivity detected with related hormones: FSH, LH and hCG
- Linearity: 1:128 dilution on 36 ?IIU/mL sample
- Precision:Â intra assay precision: CV 7.5%; inter assay precision: CV 8.0 %
- Accuracy:Â R2= 0.986 against Roche TSH Chemiluminescence Immunoassay
- Hook effect: No hook effect up to 8000 μIU/mL
- Interference: not affected by Hemoglobin 500mg/dL, Diltiazem 15uM, Bilirubin 20 mg/dL, Erythromycin 81.6uM, Triglycerides 3g/dL, Verapamil 4.4uM, HAMA 52.5ng/mL, T3 100ng/mL, RF 215IU/mL, T4 100ug/dL, Acetaminophen 1324uM
- Calibration:Â Human TSH WHO 3rd International Standards (81/565 NIBSC)
- Calibrators: 0, 0.5, 2.0, 5.0, 10, 20, 40 µIU/mL in biological matrix
- Shelf life:Â 18 months
- Assay time:Â 80 minutes
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